- 专利标题： TAILORED RECOMBINASE FOR RECOMBINING ASYMMETRIC TARGET SITES IN A PLURALITY OF RETROVIRUS STRAINS
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申请号： US13698410申请日： 2011-05-27
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公开(公告)号： US20130164271A1公开(公告)日： 2013-06-27
- 发明人: Joachim Hauber , Jan Chemnitz , Frank Buchholz , Janet Chusainow
- 申请人： Joachim Hauber , Jan Chemnitz , Frank Buchholz , Janet Chusainow
- 申请人地址： DE Muenchen DE Hamburg
- 专利权人： MAX-PLANCK-GESELLSCHAFT ZUR FOEDERUG DER WISSENSCH E.V.,HENRICH-PETTE-INSTITUT LEIBNZ-INSTITUT FOR EXPERIM VIROLOGIE-STIFTUNG B
- 当前专利权人： MAX-PLANCK-GESELLSCHAFT ZUR FOEDERUG DER WISSENSCH E.V.,HENRICH-PETTE-INSTITUT LEIBNZ-INSTITUT FOR EXPERIM VIROLOGIE-STIFTUNG B
- 当前专利权人地址： DE Muenchen DE Hamburg
- 优先权： EP10005499.8 20100527
- 国际申请： PCT/EP11/02646 WO 20110527
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N15/10
摘要：
The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.
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