发明申请
US20130164735A1 METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY 审中-公开
测量人CYP3A诱导的方法

  • 专利标题: METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY
  • 专利标题(中): 测量人CYP3A诱导的方法
  • 申请号: US13754006
    申请日: 2013-01-30
  • 公开(公告)号: US20130164735A1
    公开(公告)日: 2013-06-27
  • 发明人: Yasushi YamazoeKiyoshi Nagata
  • 申请人: Yasushi YamazoeKiyoshi Nagata
  • 优先权: JP2002-112364 20020415
  • 主分类号: C12Q1/66
  • IPC分类号: C12Q1/66
METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY
摘要:
A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells.
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