A system and a method for real-time processing and monitoring, the system including a light source to provide an illumination light and a calibration light are provided. The system includes an optical element to separate the illumination light and the calibration light; an optical element to direct the illumination light to a sample; an optical element to direct the calibration light to a first detector and a second detector; an optical element to collect light backscattered from the sample; an optical element to separate light backscattered from the sample into a first scattered light portion and a second scattered light portion; an optical element to direct the first scattered light portion through at least one multivariate optical element to the first detector; and an optical element to direct the second scattered light portion to the second detector.