The present invention relates to a technique relating to a method and apparatus for generating, transmitting, and receiving a data frame having a newly proposed format in a wireless communication system. According to the technique, the method for generating a data frame in a wireless communication system comprises the following steps: generating at least one first subframe; generating at least one second subframe; and generating a data frame including the first and second subframes, wherein the first and second subframes include length information of a MAC protocol data unit (MPDU) contained in the first and second subframes, and the length information of the MPDU contained in the second subframe is zero.