发明申请
- 专利标题： Droplet Actuator and Droplet-Based Techniques
- 专利标题（中）： 滴液致动器和基于液滴的技术
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申请号： US13390121申请日： 2010-08-13
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公开(公告)号： US20130288254A1公开(公告)日： 2013-10-31
- 发明人: Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Prasanna Thwar , Zhishan Hua , Arjun Sudarsan , Theodore Winger
- 申请人： Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Prasanna Thwar , Zhishan Hua , Arjun Sudarsan , Theodore Winger
- 申请人地址： US NC Morrisville
- 专利权人： ADVANCED LIQUID LOGIC, INC.
- 当前专利权人： ADVANCED LIQUID LOGIC, INC.
- 当前专利权人地址： US NC Morrisville
- 国际申请： PCT/US2010/045461 WO 20100813
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator. In yet another embodiment, the method of the invention combines PCR amplification with pyrosequencing to investigate specific sequences.
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