发明申请
- 专利标题: PROCESS FOR PURIFYING CYCLOLIPOPEPTIDE COMPOUNDS OR THE SALTS THEREOF
- 专利标题(中): 净化环磷脂化合物或其盐的方法
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申请号: US13877141申请日: 2011-09-27
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公开(公告)号: US20130296529A1公开(公告)日: 2013-11-07
- 发明人: Zhaoli Zhang , Shidong Liu , Zhonghao Zhuo , Xiaoming Ji
- 申请人: Zhaoli Zhang , Shidong Liu , Zhonghao Zhuo , Xiaoming Ji
- 申请人地址: CN Shanghai
- 专利权人: Shanghai Techwell Biopharmaceutical Co., Ltd.
- 当前专利权人: Shanghai Techwell Biopharmaceutical Co., Ltd.
- 当前专利权人地址: CN Shanghai
- 优先权: CN201010298554.6 20100930
- 国际申请: PCT/CN2011/080227 WO 20110927
- 主分类号: C07K7/56
- IPC分类号: C07K7/56
摘要:
A method for purifying a cyclic lipopepteide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporpous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.