A positioning structure for positioning a plate-shaped structure having at least one positioning hole is disclosed. The positioning structure includes at least one positioning element corresponding to the positioning hole, and the positioning element includes a placement portion for supporting the plate-shaped structure and a positioning portion extending toward a direction far away from the placement portion to form a hollow cylinder shape, and the positioning portion has an outer surrounding surface and a plurality of inner threads. Hence, when the outer surrounding surface of the positioning portion of the positioning element is inserted into the positioning hole to contact with the inner surface of the positioning hole, the positioning portion of the position element can be positioned in the positioning hole, thus the instant disclosure can be used to accurately position and secure the plate-shaped structure.