发明申请
US20130309747A1 MGMT-BASED METHOD FOR OBTAINING HIGH YEILDS OF RECOMBINANT PROTEIN EXPRESSION
有权
用于获得重组蛋白表达高分子的基于MGMT的方法
- 专利标题： MGMT-BASED METHOD FOR OBTAINING HIGH YEILDS OF RECOMBINANT PROTEIN EXPRESSION
- 专利标题（中）： 用于获得重组蛋白表达高分子的基于MGMT的方法
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申请号： US13824476申请日： 2011-12-09
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公开(公告)号： US20130309747A1公开(公告)日： 2013-11-21
- 发明人: Philippe Despres , Sylvie Paulous , Elodie Crublet
- 申请人： Philippe Despres , Sylvie Paulous , Elodie Crublet
- 优先权： EP10306389.7 20101209
- 国际申请： PCT/EP11/72387 WO 20111209
- 主分类号： C12N9/96
- IPC分类号： C12N9/96
摘要：
The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.
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