A display apparatus with a display panel is provided. The display panel is initially driven to have the data voltages on any two consecutive data lines thereon with different polarities. If a to-be-displayed image contains a predetermined pattern constituted by pixels in row and two adjacent pixels therein have a gray-level difference therebetween greater than a predetermined value, a timing control circuit of the display apparatus divides the associated data lines into a plurality of data line groups each constituted by four consecutive data lines and configure the data voltages on the two middle data lines in one data line group to have a first polarity and the data voltages on the rest two data lines in the same data line group to have a second polarity. An operation method of the display apparatus is also provided.