发明申请
- 专利标题： MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS
- 专利标题（中）： B细胞EBV转化的单克隆抗体生产
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申请号： US13870804申请日： 2013-04-25
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公开(公告)号： US20140004123A1公开(公告)日： 2014-01-02
- 发明人: Antonio Lanzavecchia
- 申请人： Institute For Research in Biomedicine
- 申请人地址： CH Bellinzona
- 专利权人： Institute For Research in Biomedicine
- 当前专利权人： Institute For Research in Biomedicine
- 当前专利权人地址： CH Bellinzona
- 优先权： GB0304363.5 20030226; GB0318431.4 20030806; IBPCT/IB2004/001071 20030806; GB0325391.1 20031030
- 主分类号： C07K16/10
- IPC分类号： C07K16/10 ; C12P21/00
摘要：
A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Ban virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity.
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