A barcode reading method and a barcode reading device are provided. An image sensor is utilized to capture an image of a barcode via an optical lens, and then a triangulation method and an ideal lens imagining method are collocated to calculate the digital image so as to produce an optimum image distance. A distance between the image sensor and the optical lens is then adjusted in accordance with the optimum image distance, so that the barcode can be immediately and clearly imaged on the image sensor.