发明申请
- 专利标题： NOVEL COMBINATION OF FLUORESCENT DYES FOR THE DETECTION OF NUCLEIC ACIDS
- 专利标题（中）： 用于检测核酸的荧光染料的新组合
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申请号： US14091804申请日： 2013-11-27
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公开(公告)号： US20140087956A1公开(公告)日： 2014-03-27
- 发明人: Werner Brabetz , Cornelia Weber
- 申请人： QIAGEN GMBH
- 申请人地址： DE Hilden
- 专利权人： QIAGEN GMBH
- 当前专利权人： QIAGEN GMBH
- 当前专利权人地址： DE Hilden
- 优先权： EP08170406.6 20081201; EP09156749.5 20090330
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL.
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