发明申请
US20140186828A1 METHODS FOR DETERMINING CELL VIABILITY USING MOLECULAR NUCLEIC ACID-BASED TECHNIQUES
审中-公开
使用基于分子核酸的技术测定细胞毒性的方法
- 专利标题： METHODS FOR DETERMINING CELL VIABILITY USING MOLECULAR NUCLEIC ACID-BASED TECHNIQUES
- 专利标题（中）： 使用基于分子核酸的技术测定细胞毒性的方法
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申请号： US13977719申请日： 2011-12-27
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公开(公告)号： US20140186828A1公开(公告)日： 2014-07-03
- 发明人: Shawn Mark O'Hara
- 申请人： Shawn Mark O'Hara
- 国际申请： PCT/US11/67329 WO 20111227
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention relates to novel methods, and kits, for selectively excluding dead cells from a mixture containing live and dead cells, such as microbe cells in clinical samples, blood products, medical/biotechnology products and food products where subsequent interrogation of the selected live cells are an indicator of the presence of microbe viability. In particular, the invention relates to improved methods for performing direct nucleic acid amplification techniques such as Polymerase Chain Reaction (PCR) and isothermal techniques in blood and other body fluids, for correlation with microbe cell viability from Bacteremia and Fungemia samples. The improved methods provided by the invention are particularly advantageous for the diagnosis of septicemia and to determine pathological conditions in all other normally sterile body fluids.