发明申请
US20140206006A1 SINGLE CELL CLASSIFICATION METHOD, GENE SCREENING METHOD AND DEVICE THEREOF
审中-公开
单细胞分类方法,基因筛选方法及其装置
- 专利标题: SINGLE CELL CLASSIFICATION METHOD, GENE SCREENING METHOD AND DEVICE THEREOF
- 专利标题(中): 单细胞分类方法,基因筛选方法及其装置
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申请号: US14239650申请日: 2012-08-24
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公开(公告)号: US20140206006A1公开(公告)日: 2014-07-24
- 发明人: Xun Xu , Li Bao , Weiming He , Yong Hou , Ye Tao
- 申请人: Xun Xu , Li Bao , Weiming He , Yong Hou , Ye Tao
- 申请人地址: CN Guangdong CN Guangdong
- 专利权人: BGI SHENZHEN,BGI SHENZHEN CO., LIMITED
- 当前专利权人: BGI SHENZHEN,BGI SHENZHEN CO., LIMITED
- 当前专利权人地址: CN Guangdong CN Guangdong
- 优先权: CN201110245356.8 20110825
- 国际申请: PCT/CN2012/080578 WO 20120824
- 主分类号: G06F19/22
- IPC分类号: G06F19/22
摘要:
Provided are a single cell classification method, a gene screening method and a device for implementing the method. In that, the single cell classification method includes the following steps: sequencing the whole genomes of a plurality of single cell samples from the same group, respectively, so as to obtain reads from each single cell sample; aligning the reads from each single cell sample to the sequence of a reference genome, respectively, and performing data filtering on said reads; on the basis of the filtered reads, determining a consistent genotype of each single cell sample, in which consistent genotypes of all the single cell samples constitute an SNP dataset of said group; aimed at said each single cell, on the basis of the SNP dataset of said group, determining a corresponding genotype for each cell at a site corresponding to a position in an SNP dataset of the reference genome; and selecting an SNP site associated with cell mutation, and on the basis of the genotype of said single cell at the site, classifying said single cell.
公开/授权文献
- US2682373A High-speed counter 公开/授权日:1954-06-29
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