发明申请
- 专利标题： Primer Set and Method for Homologous Recombination
- 专利标题（中）： 引物组和同源重组方法
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申请号： US14123351申请日： 2012-06-01
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公开(公告)号： US20140206084A1公开(公告)日： 2014-07-24
- 发明人: Yutaka Nakashimada , Akihisa Kita , Tohru Suzuki , Shinsuke Sakai , Kazue Takaoka
- 申请人： Yutaka Nakashimada , Akihisa Kita , Tohru Suzuki , Shinsuke Sakai , Kazue Takaoka
- 申请人地址： JP Tokyo JP Gifu JP Hiroshima
- 专利权人： MITSUI ENGINEERING & SHIPBUILDING CO., LTD.,GIFU UNIVERSITY,HIROSHIMA UNIVERSITY
- 当前专利权人： MITSUI ENGINEERING & SHIPBUILDING CO., LTD.,GIFU UNIVERSITY,HIROSHIMA UNIVERSITY
- 当前专利权人地址： JP Tokyo JP Gifu JP Hiroshima
- 优先权： JPJP2011-124459 20110602
- 国际申请： PCT/JP2012/064307 WO 20120601
- 主分类号： C12N15/90
- IPC分类号： C12N15/90 ; C12N15/74
摘要：
The present invention provides a primer set used for transformation that imparts a uracil requiring property by deleting or destroying a gene coding for orotidine-5-phosphate decarboxylase in Moorella bacteria. The present invention is accomplished by a primer set that is used for creating a uracil requiring strain obtained by deleting or destroying a gene coding for orotidine-5-phosphate decarboxylase in Moorella bacteria by homologous recombination and is represented by SEQ ID No. 1 and 2 that amplify an upstream region adjacent to said gene coding for orotidine-5-phosphate decarboxylase.