Invention Application
- Patent Title: METHOD FOR DETECTING TOXIN-PRODUCING CLOSTRIDIUM DIFFICILE
- Patent Title (中): 检测产生毒素的方法
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Application No.: US14239409Application Date: 2012-08-31
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Publication No.: US20140212879A1Publication Date: 2014-07-31
- Inventor: Hiroyuki Kubota , Hiroshi Makino , Takafumi Sakai , Eiji Ishikawa , Kenji Oishi
- Applicant: Hiroyuki Kubota , Hiroshi Makino , Takafumi Sakai , Eiji Ishikawa , Kenji Oishi
- Applicant Address: JP Minato-ku
- Assignee: KABUSHIKI KAISHA YAKULT HONSHA
- Current Assignee: KABUSHIKI KAISHA YAKULT HONSHA
- Current Assignee Address: JP Minato-ku
- Priority: JP2011-190706 20110901
- International Application: PCT/JP12/72219 WO 20120831
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
Provided are an oligonucleotide which realizes specific and highly sensitive detection of toxigenic C. difficile, and a method for detecting toxigenic C. difficile, the method employing the oligonucleotide.1) A primer pair containing an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 1, and an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 2; or a primer pair having complementary sequences to the nucleotide sequences. 2) A primer pair containing an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 4, and an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 5; or a primer pair having complementary sequences to the nucleotide sequences.
Public/Granted literature
- US09388474B2 Method for detecting toxin-producing Clostridium difficile Public/Granted day:2016-07-12
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