A ridged cylinder is fused to a sheet of filter material that extends to form a pouch. When the cylinder is placed into the opening of a vessel of a matched diameter, it creates a barrier via the filter material, through which all materials must pass to reside in the vessel. The mammal's penis is directed to ejaculate into the cylinder, and the ejaculate plug is retained by the filter material, while the semen passes through the filter and into the vessel. When the collection is complete, the cylinder and attached filter material are removed from the vessel and disposed of.