Provided is a reflection detection type measurement apparatus for skin fluorescence. The apparatus includes: a first light source irradiating excitation light on a measurement target; a second light source irradiating light of a wavelength different from that of light from the first light source on the measurement target; a first optical detector detecting a reflected light generated from the measurement target due to the excitation light of the first light source; and a second optical detector detecting a skin fluorescence generated from the measurement target due to the excitation light of the first light source, and detecting a reflected light generated from the measurement target due to the excitation light of the second light source. The first optical detector is disposed in an area other than a total reflection area to which the excitation light of the first light source is reflected after irradiating to the measurement target.