A wavelength conversion member, a backlight assembly, and a display device including the same are provided. In one aspect, there is provided a wavelength conversion member comprising a first substrate, a second substrate, a wavelength conversion layer, and a spacer. The second substrate is positioned on the first substrate. The wavelength conversion layer is interposed between the first substrate and the second substrate. The spacer is interposed between the first substrate and the second substrate to constantly keep a distance between the first substrate and the second substrate.