A three-dimensional prototyping composition includes a construction powder mixture and an inkjet liquid. The construction powder mixture includes a powdery molding material and a powdery binding agent. The inkjet liquid includes a nonionic surfactant, at least one polyalcohol compound, an alcohol ether compound, an antimicrobial agent and deionized water. While the inkjet liquid and the construction powder mixture are in contact with each other, the physical binding function of the powdery binding agent of the construction powder mixture is activated. Consequently, the powdery molding material and the powdery binding agent stick together to form a molded construction layer of a three-dimensional object. Moreover, the construction powder mixture can be recycled. Moreover, since the inkjet liquid may be ejected through the thermal bubble printhead, the fabricating cost of the three-dimensional physical model is reduced.