A uniflow two-stroke engine, includes: a cylinder receiving a piston such that the piston can reciprocate therein; an exhaust port provided in an upper end part of the cylinder; and a scavenging port that is provided in a lower side wall portion of the cylinder so as to be opened and closed by the piston, wherein a downstream portion of the scavenging port that opens out to the cylinder includes a guide member that gives a downward velocity component to a gas flow entering the cylinder from the scavenging port.