The present invention provides a positioning and adjusting device of the monitor, comprising a supporting frame, the main body of the monitor is installed on a supporting frame in an inclined way, the rear seat is hinged to the upper end of the supporting frame, the display panel is in sliding connection with the rear seat; a convex part is disposed on the backside and extended into the inner cavity of the rear seat, a convex pin is disposed next to the side of the convex part, and a hook is set on the inner cavity of the rear seat that makes the display panel in a downward positioning status, when the convex pin is moving upward along the display panel. The device can adjust the vertical position of the display panel and adjust the tilt angle of the display panel to meet the watching needs of consumers.