The present disclosure relates to a method of preparing an antimicrobial glass, which includes: preparing a glass substrate; preparing a dry paste; selectively coating the dry paste at least a portion of the glass substrate; heat-treating the coated glass substrate after the coating; and cleaning the heat -treated glass substrate, wherein the preparation of the dry paste includes preparing a diluted solution by diluting a salt including silver ions or copper ions in water, and adding a metal oxide to the diluted solution, and an antimicrobial glass prepared according to the method.