The present invention discloses a pixel structure and a manufacturing method thereof, a light-emitting device, an array substrate and a display device. The pixel structure comprises a plurality of pixel units sequentially arranged, each pixel unit comprising a plurality of color sub-pixel units, wherein the color sub-pixel unit of a certain color to which human eyes have poor discriminating power is positioned in a central position of the pixel unit, and the color sub-pixel units of the remaining colors are positioned around the color sub-pixel unit of the certain color, and an area of the color sub-pixel unit of the certain color is larger than that of any one of the color sub-pixel units of the remaining colors.