A sampling assembly configured to be coupled to a sample source is provided. The sampling assembly is configured to facilitate aseptic sampling at one or more instances in time. The sampling assembly includes a first conduit having a first port and a second port, where the first port is configured to be coupled to the sample source, and where the second port is configured to be hermetically sealed. The sampling assembly further includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connection points, and where each of the sub-ports is in fluidic communication with the first conduit. Moreover, the sampling assembly includes a plurality of sampling kits, where each sampling kit of the plurality of sampling kits is operatively connected to a respective sub-port of a corresponding sub-conduit.