发明申请
US20160053298A1 METHODS FOR IDENTIFYING PROTEINS AND COMPOUNDS THAT MODULATE THE ACTIVITY OF OTUB1
审中-公开
用于鉴定调节OTUB1活性的蛋白质和化合物的方法
- 专利标题： METHODS FOR IDENTIFYING PROTEINS AND COMPOUNDS THAT MODULATE THE ACTIVITY OF OTUB1
- 专利标题（中）： 用于鉴定调节OTUB1活性的蛋白质和化合物的方法
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申请号： US14785429申请日： 2014-04-14
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公开(公告)号： US20160053298A1公开(公告)日： 2016-02-25
- 发明人: Cynthia Wolberger , Anthony Dibello , Reuven Wiener
- 申请人： The Johns Hopkins University
- 申请人地址： US MD Baltimore
- 专利权人： The Johns Hopkins University
- 当前专利权人： The Johns Hopkins University
- 当前专利权人地址： US MD Baltimore
- 国际申请： PCT/US2014/033947 WO 20140414
- 主分类号： C12Q1/37
- IPC分类号： C12Q1/37
摘要：
The present invention describes that OTUB1 cleavage of K48 poly-ubiquitin is stimulated by a select subset of E2 enzymes, and that this stimulation is regulated by the ubiquitin-charged state of the E2 and free ubiquitin. Structural and biochemical studies of OTUB1 and UBCH5B show that the E2 stimulates binding of the polyubiquitin substrate by contacting the OTUB1 N-terminal ubiquitin-binding helix. Methods for identifying E2 enzymes which stimulate or inhibit cleavage of K48 polyubiquitin, as well as novel target compounds which modulate this interaction are provided.
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