发明申请
US20160060643A1 PREMETHYLATION OF DNA FOR HIGH EFFICIENCY TRANSFORMATION OF CYANOBACTERIA
审中-公开
用于高效转染细胞因子的DNA的预处理
- 专利标题： PREMETHYLATION OF DNA FOR HIGH EFFICIENCY TRANSFORMATION OF CYANOBACTERIA
- 专利标题（中）： 用于高效转染细胞因子的DNA的预处理
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申请号： US14842671申请日： 2015-09-01
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公开(公告)号： US20160060643A1公开(公告)日： 2016-03-03
- 发明人: Bo Wang , Weiwen Zhang , Deirdre Meldrum
- 申请人： Bo Wang , Weiwen Zhang , Deirdre Meldrum
- 申请人地址： US AZ Scottsdale
- 专利权人： Arizona Board of Regents on behalf of Arizona State University
- 当前专利权人： Arizona Board of Regents on behalf of Arizona State University
- 当前专利权人地址： US AZ Scottsdale
- 主分类号： C12N15/82
- IPC分类号： C12N15/82
摘要：
Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome of Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) and expressed in E. coli that harbors the integrative plasmid pBS-SPtK or pJU105. After pre-methylation in E. coli, the integrative plasmids were extracted and used for transformation of Synechocystis 6803. The results showed that expression of slr0214 in the integrative-plasmid-harboring E. coli cells before DNA preparation resulted in orders of magnitude higher efficiency in the following integrative transformation of Synechocystis 6803.
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