A stacked type image sensor including color separation elements, and an image pickup apparatus including the stacked type image sensor, are provided. The stacked type image sensor includes a first light sensing layer including first pixels configured to absorb and detect light of a first wavelength band and transmit light of a second wavelength band and a third wavelength band, and a second light sensing layer disposed to face the first light sensing layer, the second light sensing layer including second pixels configured to detect light of the second wavelength band and third pixels configured to detect light of the third wavelength band. The color separation elements are disposed between the first light sensing layer and the second light sensing layer, and are configured to direct the light of the second wavelength band toward the second pixels, and direct the light of the third wavelength band toward the third pixels.