A vessel sensing device with automatic amendment function includes an analog processing circuit, a signal generating circuit and a DC voltage detecting circuit. The analog processing circuit includes an optical sensing module. An optical sensor of the optical sensing module generates an analog signal with a skin characteristic signal and a vessel characteristic signal according to an optical reflecting signal. The skin characteristic signal and the vessel characteristic signal respectively correspond to a skin feature and a vessel feature of the user. The signal generating circuit is coupled to the analog processing circuit to transform the analog signal into a compensation signal. The DC voltage detecting circuit is coupled to the signal generating circuit and adapted to compensate the analog processing circuit according to the compensation signal, so as to decrease the skin characteristic signal within the analog signal.