发明申请
- 专利标题: METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS
- 专利标题(中): 直接化学分析的方法和组合
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申请号: US15067428申请日: 2016-03-11
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公开(公告)号: US20160194687A1公开(公告)日: 2016-07-07
- 发明人: Feng Yang , Sha-Sha Wang , Laurence Michael Vaughan , Michael Porter , Elaine Rose
- 申请人: Becton, Dickinson and Company
- 申请人地址: US NJ Franklin Lakes
- 专利权人: Becton, Dickinson and Company
- 当前专利权人: Becton, Dickinson and Company
- 当前专利权人地址: US NJ Franklin Lakes
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N33/569 ; C12Q1/70 ; C12N1/06
摘要:
A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.
公开/授权文献
- US10323267B2 Methods and compositions for direct chemical lysis 公开/授权日:2019-06-18