A display substrate (20) includes: a blue phase liquid crystal layer (25) and one or more sets of driving electrodes for driving the blue phase liquid crystal layer (25) in dark state; each set of the driving electrodes include two hetero-potential electrodes (21, 22), and the two hetero-potential electrodes (21, 22) generate an electric field at the blue phase liquid crystal layer (25) with a non-zero component in a first direction that is perpendicular to the polarization direction of incident light. This display substrate can reduce dark state light leakage and enhance display quality of devices. A manufacturing method for the display substrate (20) and a display device are disclosed.