A 3D display driving method and a 3D glasses driving method are provided. In the 3D display driving method, after a left-eye image and a right-eye image in a stereoscopic image pair of each frame are displayed, one frame of buffer image is inserted, then a left-eye image and a right-eye image in a stereoscopic image pair of a next frame are displayed. By inserting one frame of buffer image between two consecutive frames of left-eye and right-eye image pairs, the dizziness and blurring feeling generated due to the visual persistence characteristics of the human eyes when the human brain processes different image pairs are relieved.