Embodiments of this disclosure provide a transparent display panel and a manufacturing method thereof, and a display device. The transparent display panel comprises a plurality of light-emitting regions and a plurality of transparent regions. The transparent display panel further comprises at least one light-guiding component disposed on a light-emitting side of the transparent display panel, wherein the at least one light-guiding component is configured to transmit a part of light emitted from the light-emitting regions to the transparent regions. In the transparent display panel, a light-guiding component is used to transmit the light emitted by the light-emitting subpixels in the light-emitting regions to the transparent regions, and a plurality of light-redirecting members formed on a surface of the light-guiding component are used to change the direction of light transmitted to the transparent regions and to emit the light from the light-emitting side of the transparent regions. As a result, display uniformity of the whole transparent display panel is improved, and a better display performance can be achieved.