The present disclosure relates to the field of display technology, and provides a display substrate, its driving method and a display device, so as to reduce the power consumption of the display device. The display substrate includes a plurality of first transparent regions arranged in an array form. Each first transparent region serves as a subpixel, and a pixel includes at least two adjacent subpixels. The subpixels in each pixel correspond to backlight lamps in different colors respectively. The display substrate further includes a plurality of light-shielding structures each corresponding to one of the subpixels and configured to adjust a light transmission rate of the subpixel.