The embodiments of the present invention disclose an electrochromic grating, a manufacturing method thereof, a display panel and a display device. The method of manufacturing the electrochromic grating comprises: forming a first transparent electrode layer on a first substrate; forming a resin layer on the first transparent electrode layer and forming a plurality of grooves in the resin layer; forming a second transparent electrode layer on a second substrate; perfusing at least one of solution type or gel type electrochromic material into the plurality of grooves; and then, encapsulating the first substrate and the second substrate. In this way, by using the at least one of solution type or gel type electrochromic material filled in the grooves, the conversion speed of the electrochromic grating between the transparent state and the colored state can be increased, the conversion response speed of the display panel between 2D picture and 3D picture can be further increased, so as to realize a higher contrast, thereby improving the display effect.