An object holder assembly in a vehicle that includes a floor having an upper face intended to support at least one object and at least one trunk separator having a base able to be applied on the floor and a separating partition. The separator includes a fastening device having an active configuration in which the fastening device is able to fasten the base on the floor when the base is in contact with the upper face of the floor and a released configuration of the separator, and a control member for the fastening device, able to be actuated between a locking position, in which the control member activates the fastening device, and an unlocking position.