发明申请
- 专利标题: METHOD FOR MANUFACTURING CILIARY MARGINAL ZONE-LIKE STRUCTURE
- 专利标题(中): 制造军事区域结构的方法
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申请号: US15103162申请日: 2014-10-16
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公开(公告)号: US20160376554A1公开(公告)日: 2016-12-29
- 发明人: Atsushi KUWAHARA , Yoshiki SASAI
- 申请人: SUMITOMO CHEMICAL COMPANY, LIMITED , RIKEN
- 申请人地址: JP Tokyo JP Wako-shi
- 专利权人: SUMITOMO CHEMICAL COMPANY, LIMITED,RIKEN
- 当前专利权人: SUMITOMO CHEMICAL COMPANY, LIMITED,RIKEN
- 当前专利权人地址: JP Tokyo JP Wako-shi
- 优先权: JP2013-255836 20131211
- 国际申请: PCT/JP2014/077602 WO 20141016
- 主分类号: C12N5/079
- IPC分类号: C12N5/079 ; G01N33/50 ; A61K9/00 ; A61L27/36 ; A61K35/30 ; A61L27/38
摘要:
The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more and 100% or less of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway and a substance inhibiting the FGF signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene-expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium each free of a substance acting on the Wnt signal pathway and so on. According to the production method of the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.