The present disclosure provides a heat dissipation device, a working method thereof and a display device. The heat dissipation device is applied to a display device, including a temperature monitoring unit, configured to monitor an environment temperature of each predetermined region of the display device; a control unit, connected to the temperature monitoring unit, configured to determine regions to be cooled according to a monitoring result of the temperature monitoring unit and then send a control instruction to a movement unit; the movement unit, connected to the control unit, and configured to move at least one heat dissipation fan to the regions to be cooled in response to the received control instruction; and the at least one heat dissipation fan connected to the movement unit.