A display substrate, a method for fabricating the same, and a display device are disclosed. The display substrate comprises a plurality of pixels; and a plurality of slit patterns, which are arranged between at least two of the plurality of pixels, and comprise a plurality of slits arranged in a rubbing direction. Slit patterns are provided, and each of slit patterns comprises slits in the rubbing direction. Thus, during a rubbing alignment process, the slit patterns can guide a rubbing cloth to move in the rubbing direction. Accordingly, the alignment of the rubbing cloth is prevented from changing in the rubbing process, a good alignment layer is formed, rubbing Mura is avoided, and the lifetime of the rubbing cloth is extended.