The invention provides a method for anticoagulation, including administering to a subject in need a therapeutically effective amount of pharmaceutical composition including formula (I) or its isomers as its parent form, and its salt, ester or solvate. The invention also provides a method for anticoagulation, including administering to a subject in need a therapeutically effective amount of pharmaceutical composition including formula (I) or its isomers as its parent form, and its salt, ester or solvate for at least 1 hour, wherein a dose of the pharmaceutical composition is in an amount of about 5×10−5 mL/kg or more of body weight of the subject.