A touch substrate comprises a first substrate, a second substrate, a first conductive layer and a second conductive layer. When the second substrate is not subjected to a pressure, the second conductive layer is insulatively spaced apart from the first conductive layer; when the second substrate is subjected to a pressure equal to or greater than a predetermined value, the second substrate is deformed so that at least a part of the second conductive layer is contacted with the first conductive layer. A touch display panel and a display device which comprise the touch substrate are also provided.