A printing screen and a sealant printing method are provided. The printing screen includes a frame, a mesh/screen fixed on the frame, and a film formed on the mesh. The portion of the mesh that is not covered by the film forms a feeding port in a rectangular ring shape. The rectangular ring includes two opposite first sides and two opposite second sides. The width of an upper opening of the cross section across the first sides is less than or equal to that of an upper opening of the cross section across the second sides. At a first height, the area of the cross section across the first sides is greater than that of the cross section across the second sides, the first height being less than the thickness of the film. The printing screen and the sealant printing method solve the problem of poor height uniformity of sealants.