A method and device for positioning human eyes are disclosed. The method includes: acquiring an input image; performing grayscale processing to the image to extract a grayscale feature; extracting a candidate human eye area in the image by employing a center-periphery contrast filter algorithm according to the grayscale feature; extracting left and right eye candidate areas respectively from the candidate human eye area through a pre-created human eye statistical model; and checking pairing on the left and right eye candidate areas to determine positions of left and right eyes.