A screen plate and a manufacturing method of the screen plate, provided for solving a problem of glass cement being thicker at the center and thinner at the border during printing glass cement by use of the screen plate in the prior arts and an inclination of burr generated at the borders. In the screen plate, a pattern layer comprises a plurality of openings, each of which comprises at least two sub-openings which have horizontal dimensions gradually decreasing in a vertical direction from a side close to the screen to a side away from the screen, so that a blade can squeeze the glass cement through a region of the screen corresponding to the sub-opening closest to the screen and further squeeze the glass cement through the plurality of the sub-openings having gradually reduced horizontal dimensions, thereby achieving a uniform output amount of the glass cement at the sub-opening farthest from the screen and eliminating the burr at the border of the printed pattern of the glass cement.