发明申请
- 专利标题: BLOOD SAMPLE ASSAY METHOD
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申请号: US15629672申请日: 2017-06-21
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公开(公告)号: US20170349933A1公开(公告)日: 2017-12-07
- 发明人: Arne Ludvig FAAREN , Frank FRANTZEN , Arne Kristian NORDHEI , Erling SUNDREHAGEN , Lars ØRNING
- 申请人: AXIS-SHIELD AS
- 申请人地址: NO Oslo
- 专利权人: AXIS-SHIELD AS
- 当前专利权人: AXIS-SHIELD AS
- 当前专利权人地址: NO Oslo
- 优先权: GB1119515.3 20111111; GB1201154.0 20120124; GB1201245.6 20120125
- 主分类号: C12Q1/60
- IPC分类号: C12Q1/60 ; G01N33/92 ; C12Q1/61
摘要:
The invention provides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The reagent of step iii) may be applied to the sample separately or may be included in a reagent mixture during steps i) or iv). A corresponding kit is also provided.