The present invention discloses a nano-conductive rubber sensing unit and a preparation method therefor, which belong to the technical field of pressure measurement. The nano-conductive rubber sensing unit of the invention comprises at least two fabric layers, wherein nano-conductive rubber is filled between every two adjacent fabric layers, and the nano-conductive rubber is a rubber matrix in which carbon nanotubes are dispersed. The preparation method for the nano-conductive rubber sensing unit of the invention comprises: S1, mixing a rubber matrix with carbon nanotubes in accordance with a mass proportion so as to make a nano-conductive rubber slurry; S2, laying flat one fabric layer, spreading the nano-conductive rubber slurry prepared in S1 over the fabric uniformly to a certain thickness, and then, laying flat the other fabric layer thereon; and S3, pressurizing and heating the nano-conductive rubber sensing unit prepared in S2 to cure the same. The nano-conductive rubber sensing unit of the invention achieves the technical effects of a large measuring range of pressure measurement, high sensitivity in the measuring range and good linearity of a piezoresistance characteristic curve, and can meet the requirement of a sheet type.