The embodiments of the present invention provide a display substrate, a method for manufacturing the same and a liquid crystal display device. The display substrate includes a display region and a non-display region. The non-display region includes at least a sealing region surrounding the display region. The sealing region includes a segment difference padding layer, and the segment difference padding layer includes several through holes. The display substrate, the method for manufacturing the same and the liquid crystal display device provided by the embodiments of the invention can eliminate the mura phenomenon, simplify the process and prevent the resin layer from being peeled off from the substrate.