Invention Application
- Patent Title: ALKYLENE GLYCOLS AND POLYMERS AND COPOLYMERS THEREOF FOR DIRECT ISOLATION OF NUCLEIC ACID FROM EMBEDDED SAMPLES
-
Application No.: US15829469Application Date: 2017-12-01
-
Publication No.: US20180087048A1Publication Date: 2018-03-29
- Inventor: Richard CONRAD , Marie Gonzalez , Emily ZERINGER
- Applicant: Life Technologies Corporation
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12Q1/68

Abstract:
Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffinization step prior to isolating nucleic acids from paraffin-embedded samples, for example.
Information query
IPC分类: