The present application discloses a touch substrate and a manufacturing method thereof, and a touch screen. The touch substrate has a touch area and a function-hole area and includes a base substrate and a light shielding pattern which defines the touch area and the function-hole area. The touch substrate further includes a touch unit and a first blanking pattern arranged in the touch area and a first insulating layer, a second blanking pattern and a transmission enhancement pattern arranged in the function-hole area. The first blanking pattern and the second blanking pattern are arranged in a same layer, and the transmission enhancement pattern is configured to increase a light transmittance of an area corresponding to the second blanking pattern.