The present disclosure relates to a microfluidic control system and a microfluidic control method using the same. The microfluidic control system includes: a microfluidic chip including a storage chamber for storing a reaction solution and a receiving chamber communicating with the storage chamber; and a microfluidic control device for controlling the reaction solution inside the microfluidic chip, wherein the microfluidic control device includes: a first roller which is in contact with the microfluidic chip and rotates together with the movement of the microfluidic chip; and a pressurizing protrusion formed on the outer peripheral surface of the first roller, wherein the pressurizing protrusion has a shape corresponding to the storage chamber.